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KMID : 0363620100310060047
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Journal of Korean Oriental Medicine
2010 Volume.31 No. 6 p.47 ~ p.57
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Ethanol Extract of Cynanchum wilfordii Produces Endothelium-Dependent Relaxation in Rat Aorta and Anti-inflammatory Activity in Human Aortic Smooth Muscle Cells
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Choi Deok-Ho
Lee Yun-Jung
Kim Eun-Joo
Li Xiang
Kim Hye-Yoom
Hwang Sun-Mi
Yoon Jung-Joo
Lee So-Min
Min Eun-Kyeong
Kang Dae-Gill
Lee Ho-Sub
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Abstract
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Objective: The present study investigated the effect of ethanol extract of Cynanchum wilfordii (ECW) on vascular relaxation and vascular inflammation in rat artery isolated from rats and anti-inflammatory activity in human aortic smooth muscle cells (HASMC).
Methods: Vascular tone and guanosine 3’,5’-cyclic monophosphate (cGMP) production were examined in rat artery isolated from Sprague Dawley rats, in the presence of ECW. HASMC were incubated with tumor necrosis factor-alpha (TNF-) or Angiotensin II for 24 h. Matrix metalloproteinase (MMP)-2 and anti-oxidant activity of ECW was investigated by pretreatment with ECW in HASMC.
Results: Cumulative treatment of ECW relaxed aortic smooth muscles of rats in a dose-dependent manner. ECW-induced vasorelaxation was significantly decreased by pretreatment of L-arginine methyl ester (L-NAME) or oxadiazolo-quinoxalinone (ODQ). Furthermore, ECW treatment of thoracic aorta significantly increased cGMP production. Incubation of ECW with ODQ or L-NAME markedly decreased ECW-induced cGMP production. ECW treatment dose-dependently suppressed TNF-- or Angiotensin II-induced increase in matrix metalloproteinase-2 expression in HASMC. Also, ECW exhibited 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity in vitro and reduced TNF--induced increase in reactive oxygen species production in a dose-dependent manner.
Conclusions: Taken together, the results suggest that ECW exerts vascular relaxation via NO/cGMP signaling pathway and decreases MMP-2 expression via anti-oxidant activity.
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KEYWORD
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Cynanchum wilfordii, vascular relaxation, NO, cGMP, anti-oxidant activity, matrix metalloproteinase-2
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